The analysis of mini-STR loci represents a powerful tool for genetic typing of degraded DNA samples. Since the forensic application of genetic markers requires reference databases, a population sample from North-East Italy was tested at the mini-STR loci D10S1248, D14S1434 and D22S1045. DNA was extracted from fresh blood samples collected from 100 healthy unrelated subjects living in that area. The genetic typing was performed amplifying each STR marker in a single PCR. The amplicons were separated through acrylamide gels. The alleles were scored by comparison with an allelic ladder created by mixing up sequenced alleles. Allele nomenclature was performed according to Coble and Butler [2]. Allele frequencies and other forensic parameters (HE, PD, PIC, PE and PPI) are provided. No Hardy–Weinberg disequilibrium was found. Our results are in agreement with those previously found in other Italian population samples.
Allele frequencies of three mini-STR loci (D22S1045, D14S1434 and D10S1248) in North-East Italy
PREVIDERE', CARLO;
2009-01-01
Abstract
The analysis of mini-STR loci represents a powerful tool for genetic typing of degraded DNA samples. Since the forensic application of genetic markers requires reference databases, a population sample from North-East Italy was tested at the mini-STR loci D10S1248, D14S1434 and D22S1045. DNA was extracted from fresh blood samples collected from 100 healthy unrelated subjects living in that area. The genetic typing was performed amplifying each STR marker in a single PCR. The amplicons were separated through acrylamide gels. The alleles were scored by comparison with an allelic ladder created by mixing up sequenced alleles. Allele nomenclature was performed according to Coble and Butler [2]. Allele frequencies and other forensic parameters (HE, PD, PIC, PE and PPI) are provided. No Hardy–Weinberg disequilibrium was found. Our results are in agreement with those previously found in other Italian population samples.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
