Ethnopharmacological relevance:Phyllanthusmuellerianus (Kuntze)Excel(family Euphorbiaceae) stem bark isusedinCameroonbyBakapygmiesasaremedyforwoundhealingandtetanus. Aim ofthestudy: To characterizethechemicalcompositionandtoevaluatetheantimicrobialproperties of theessentialoiloftheplant. Materialsandmethods: The essentialoilwasextractedfromthestembarkbydynamicheadspaceand by hydrodistillationandcharacterizedbyGCandGC-MSanalyses.Theantimicrobialactivitywas evaluatedonthebasisoftheminimuminhibitoryconcentration(MIC)andtheminimumbactericidal– fungicidalconcentration(MBC–MFC)bythemicroandmacrodilutionmethods.Thefollowingbacteria and fungiwereused: Clostridiumsporogenes ATCC 3584, Staphylococcusaureus ATCC 6538, Streptococcus mutans ATCC 25175, Streptococcuspyogenes ATCC 19615, Escherichiacoli ATCC10536, Candidaalbicans ATCC10231, Candidaalbicans LM 450, Trichophytonmentagrophytes LM 230, Trichophytonrubrum LM 237, Microsporumcanis LM 324. Results: The hydrodistillationafforded0.06%(dryweightbasis)ofpaleyellowoil.Thirty-eight compoundsrepresenting90.69%wereidentified.Themajorcomponent(36.40%)wasfoundtobe (E)-isoelemicin,identifiedbycomparisonofits 1H-NMR experimentaldata,withliteraturedata.Theoil showedgoodantibacterialactivityagainst ClostridiumSporogenes, StreptococcusmutansandStrepto- coccuspyogenes with MICrangingfrom13.5to126 mg/ml.Aweakantifungalactivity(MIC250 mg/ml) was foundagainst Trichophytonrubrum, only. Conclusions: The antimicrobialactivityandthechemicalcompositionof Phyllanthusmuellerianus stem bark essentialoilarereportedforthefirsttime.
Chemical composition and antimicrobial activity of Phyllanthus muellerianus (Kuntze) Excel essential oil
BRUSOTTI, GLORIA;CESARI, ILARIA;GILARDONI, GIANLUCA;TOSI, SOLVEIG;GRISOLI, PIETRO;PICCO, ANNA MARIA;CACCIALANZA, GABRIELE
2012-01-01
Abstract
Ethnopharmacological relevance:Phyllanthusmuellerianus (Kuntze)Excel(family Euphorbiaceae) stem bark isusedinCameroonbyBakapygmiesasaremedyforwoundhealingandtetanus. Aim ofthestudy: To characterizethechemicalcompositionandtoevaluatetheantimicrobialproperties of theessentialoiloftheplant. Materialsandmethods: The essentialoilwasextractedfromthestembarkbydynamicheadspaceand by hydrodistillationandcharacterizedbyGCandGC-MSanalyses.Theantimicrobialactivitywas evaluatedonthebasisoftheminimuminhibitoryconcentration(MIC)andtheminimumbactericidal– fungicidalconcentration(MBC–MFC)bythemicroandmacrodilutionmethods.Thefollowingbacteria and fungiwereused: Clostridiumsporogenes ATCC 3584, Staphylococcusaureus ATCC 6538, Streptococcus mutans ATCC 25175, Streptococcuspyogenes ATCC 19615, Escherichiacoli ATCC10536, Candidaalbicans ATCC10231, Candidaalbicans LM 450, Trichophytonmentagrophytes LM 230, Trichophytonrubrum LM 237, Microsporumcanis LM 324. Results: The hydrodistillationafforded0.06%(dryweightbasis)ofpaleyellowoil.Thirty-eight compoundsrepresenting90.69%wereidentified.Themajorcomponent(36.40%)wasfoundtobe (E)-isoelemicin,identifiedbycomparisonofits 1H-NMR experimentaldata,withliteraturedata.Theoil showedgoodantibacterialactivityagainst ClostridiumSporogenes, StreptococcusmutansandStrepto- coccuspyogenes with MICrangingfrom13.5to126 mg/ml.Aweakantifungalactivity(MIC250 mg/ml) was foundagainst Trichophytonrubrum, only. Conclusions: The antimicrobialactivityandthechemicalcompositionof Phyllanthusmuellerianus stem bark essentialoilarereportedforthefirsttime.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
