Buprenorphine is a synthetic derivative of thebaine used as a substitute of heroin in detoxification pro¬ grams. Buprenorphine was introduced for this applica¬ tion in May 2000 in Italy and its use for this therapeu¬ tic purpose has increased of more than 30 % in three years. The aim of this study was to extend the method routinely applied in our laboratory to detect heroin metabolites, cocaine and amphetamines also to bupre¬ norphine (BPR) and its metabolite norbuprenorphine (norBPR) in order to monitor BPR administration in detoxification programs as well as to detect drug abuse during detoxification. The analytical procedure was as follow: after a washing-step with methanol, hair was finely cut and incubated in HC1 0.1N (45° C, over¬ night). Nalorphine was chosen as internal standard. Purification of analytes was executed by solid phase extraction (Bond Elut Certify®) which provided reco¬ veries higher than 80 % for both BPR and norBPR; purified extract was derivatised with N-methyl, N-trimethylsilyl trifluoroacetamide (MSTFA) and the ana¬ lysis was performed by GC-MS in SIM mode. Ions monitored were: m/z 450, 482, 506 for BPR, m/z 468, 500, 524 for norBPR and m/z 455, 414, 324 for inter¬ nal standard (underlined ions were used as quantifiers). Method validation was performed by: evaluation of accuracy and precision; analysis of seven drug-free hair; testing of linearity (0-0.5 ng/mg, n=5). Intra-day (n=7) and inter-day (n=3 on 5 different days) precision were better than 8.8 % for both analytes and accuracy better than 15 %. The limit of detection was 0.005 ng/mg and the limit of quantitation. was 0.01 ng/mg. This method was applied to hair samples collected from patients in withdrawal treatment program and demostrated its good applicability in routine analysis.
Determination of buprenorphine and norbuprenorphine in hair by GC-MS
STRAMESI, CRISTIANA;ZUCCHELLA, ALESSANDRA;VIGNALI, CLAUDIA MARIA;GROPPI, ANGELO;
2005-01-01
Abstract
Buprenorphine is a synthetic derivative of thebaine used as a substitute of heroin in detoxification pro¬ grams. Buprenorphine was introduced for this applica¬ tion in May 2000 in Italy and its use for this therapeu¬ tic purpose has increased of more than 30 % in three years. The aim of this study was to extend the method routinely applied in our laboratory to detect heroin metabolites, cocaine and amphetamines also to bupre¬ norphine (BPR) and its metabolite norbuprenorphine (norBPR) in order to monitor BPR administration in detoxification programs as well as to detect drug abuse during detoxification. The analytical procedure was as follow: after a washing-step with methanol, hair was finely cut and incubated in HC1 0.1N (45° C, over¬ night). Nalorphine was chosen as internal standard. Purification of analytes was executed by solid phase extraction (Bond Elut Certify®) which provided reco¬ veries higher than 80 % for both BPR and norBPR; purified extract was derivatised with N-methyl, N-trimethylsilyl trifluoroacetamide (MSTFA) and the ana¬ lysis was performed by GC-MS in SIM mode. Ions monitored were: m/z 450, 482, 506 for BPR, m/z 468, 500, 524 for norBPR and m/z 455, 414, 324 for inter¬ nal standard (underlined ions were used as quantifiers). Method validation was performed by: evaluation of accuracy and precision; analysis of seven drug-free hair; testing of linearity (0-0.5 ng/mg, n=5). Intra-day (n=7) and inter-day (n=3 on 5 different days) precision were better than 8.8 % for both analytes and accuracy better than 15 %. The limit of detection was 0.005 ng/mg and the limit of quantitation. was 0.01 ng/mg. This method was applied to hair samples collected from patients in withdrawal treatment program and demostrated its good applicability in routine analysis.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
