Caries is the most common oral infectious disease in the world. Its development is influenced also by diet components that interfere with pathogen mutans group Streptococci activity. A very active research to identify functional foods and their components that are generally recognized as safe has been ongoing, with the aim of developing alternative approaches, to the use of synthetic chlorhexidine, and at the reduction or prevention of caries. Until now convincing evidence exists only for green tea as a functional food for oral health, partly owing to its high content of catechins, especially epigallocatechin-gallate. Our previous research showed that an active Cichorium intybus fraction contained oxalic, succinic, shikimic and quinic acids. A number of tests carried out using the commercial standards confirmed their capacity in interfering with the growth and virulence traits (i.e., biofilm formation, adherence to epithelial cells and hydroxyapatite) of oral pathogens including Streptococcus mutans, Actinomyces naeslundii and Prevotella intermedia, even in different way and entity. NUTRIDENT consortium groups went on with investigation on Lentinus edodes extract. It was very active above all against caries pathogen virulence traits. Applying bio-guided purification steps, a very active fraction containing nitrogenous bases besides quinic acid was isolated. The quinic acid level was measured using a simple HILIC-UV method developed in our laboratory.
Nutraceutics in Lentinus edodes inhibiting virulence-related properties of oral pathogenic bacteria
PAPETTI, ADELE;MARRUBINI BOULAND, GIORGIO CARLO;GAZZANI, GABRIELLA
2014-01-01
Abstract
Caries is the most common oral infectious disease in the world. Its development is influenced also by diet components that interfere with pathogen mutans group Streptococci activity. A very active research to identify functional foods and their components that are generally recognized as safe has been ongoing, with the aim of developing alternative approaches, to the use of synthetic chlorhexidine, and at the reduction or prevention of caries. Until now convincing evidence exists only for green tea as a functional food for oral health, partly owing to its high content of catechins, especially epigallocatechin-gallate. Our previous research showed that an active Cichorium intybus fraction contained oxalic, succinic, shikimic and quinic acids. A number of tests carried out using the commercial standards confirmed their capacity in interfering with the growth and virulence traits (i.e., biofilm formation, adherence to epithelial cells and hydroxyapatite) of oral pathogens including Streptococcus mutans, Actinomyces naeslundii and Prevotella intermedia, even in different way and entity. NUTRIDENT consortium groups went on with investigation on Lentinus edodes extract. It was very active above all against caries pathogen virulence traits. Applying bio-guided purification steps, a very active fraction containing nitrogenous bases besides quinic acid was isolated. The quinic acid level was measured using a simple HILIC-UV method developed in our laboratory.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
